Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: Presence of intracellular vDNA from CrPV in S2 cells upon infection. Amplicons produced with CrPV-specific primers (CrPV_pair-5) from cellular DNA extracts collected immediately (T0) or three days (T3) after viral infection (V) or mock-infection (M). NTC = non-template control. The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Infection, Produced, Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: Presence of intracellular vDNA from CrPV in High Five cells upon infection. Amplicons produced with CrPV-specific primers (CrPV_pair-5) from cellular DNA extracts collected immediately (T0) or three days (T3) after viral infection (V) or mock infection (M). -: non-template control. The Thermo Fisher Scientific 1 Kb Plus DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Infection, Produced, Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: Presence of intracellular vDNA from CrPV in BmN4 cells upon infection. Amplicons produced with CrPV-specific primers (CrPV_pair-5) from cellular DNA extracts collected immediately (T0) or three days (T3) after viral infection (V) or mock infection (M). -: non-template control. The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Infection, Produced, Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: Presence of vDNA from CrPV in E. variegatus insects upon infection. Amplicons produced with CrPV-specific primers (CrPV_pair-1) from cDNA (positive control) or DNA collected four (4 dpi) or nine (9 dpi) days after viral infection (V) or mock infection (M). -: non-template control. The Thermo Fisher Scientific 1 Kb Plus DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Infection, Produced, Positive Control, Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: Presence of vDNA from CrPV in S2 EVs. Seven different primer pairs targeting the CrPV genome were used. Amplicons were produced with EV-extracted DNA from virus-infected (V) or mock-infected (M) cells immediately (T0) or 48 h/two days (T2) after treatment. RNA: RNA-only control; -: non-template control; +: positive control (cDNA). For CrPV_pair-5, one reaction (*) was performed with 7.5 ng DNA; the other reactions for this primer pair were set up with 15 ng DNA each. The Thermo Fisher Scientific 1 Kb Plus DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Produced, Virus, Infection, Control, Positive Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: vDNA from persistently present non-RT RNA viruses in lepidopteran cell lines. A: Amplicons produced with BmLV-specific primers (MLV_pair-8) from DNA, digested DNA, and RNA from B. mori BmN4 cells persistently infected with BmLV. B: Amplicons produced with BmLV-specific primers (MLV_pair-8) from DNA, RNA, and digested DNA from T. ni High Five cells persistently infected with BmLV and FHV. C: Amplicons produced with FHV-specific primers (FHV_pair-2) from T. ni High Five cells persistently infected with BmLV and FHV. -: non-template control. The Thermo Fisher Scientific 1 Kb Plus DNA Ladder was used as a reference for panels A and B, while the Thermo Fisher Scientific O’Range Ruler 200 bp DNA Ladder was used as a reference for panel C.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Produced, Infection, Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: vDNA of persistently present EVV1 in E. variegatus individuals. Amplicons produced by PCR with EVV1-specific primers from DNA of persistently infected individuals, a positive control (+ control) represented by the cDNA of one EVV1-infected insect, and an NTC (non-template control). A: EVV1-specific primers were used to amplify DNA. B: EVV1-specific primers were used to amplify RNA aliquots (treated with DNase) and ( C ) cDNA. The Thermo Fisher Scientific 1 Kb Plus DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Produced, Infection, Positive Control, Control

Journal: bioRxiv

Article Title: Infection with non-retroviral RNA viruses produces virus-derived DNA across diverse insect species

doi: 10.64898/2026.03.30.715342

Figure Lengend Snippet: Detection of viral DNA (vDNA) of persistently present H. halys partiti-like virus 1 ( P ) and H. halys toti-like virus 2 ( T ) in H. halys individuals. Left: DNA extracted from persistently infected individuals was used as a template. Right: cDNA and RNA extracted from persistently infected individuals were used as templates. -: non-template control. The Thermo Fisher Scientific 1 Kb Plus DNA ladder was used as a reference.

Article Snippet: The Thermo Fisher Scientific GeneRuler 100 bp DNA ladder was used as a reference.

Techniques: Virus, Infection, Control